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![[Yellow/Cyan] P16/Ki-67 Dual Staining Detection Kit](https://www.celnovte.com/wp-content/uploads/2025/11/9a11ed70b508d8935f98a59d6bd0f36c.jpg "[Yellow/Cyan] P16/Ki-67 Dual Staining Detection Kit")
![[Yellow/Cyan] P16/Ki-67 Dual Staining Detection Kit](https://www.celnovte.com/wp-content/uploads/2025/11/c223a7cea6fac3e8ddc916eb863712fa.jpg "[Yellow/Cyan] P16/Ki-67 Dual Staining Detection Kit")
[Yellow/Cyan] P16/Ki-67 Dual Staining Detection Kit
P16/Ki-67 dual staining detection reagent kit (immunocytochemistry) is an detection reagent for cervical cell screening shunt and diagnosis of cervical tissue samples. It adopts immunohistochemical double-staining detection and multi-antibody combination technology. Two antigen targets, p16 and Ki-97, can be detected simultaneously on the same sample tissue section by one staining process.
The p16 gene has the function of inhibiting cell proliferation during the cell cycle arrest period and is strongly expressed in cervical intraepithelial neoplasia and cervical cancer, which is an objective biomarker for cervical lesions. Ki-67 is a biomarker of cell proliferation, and its overexpression indicates that the cell is in the proliferation phase. Normally, the expressions of p16 and Ki-67 are mutually exclusive. The co-expression of p16 and Ki-67 indicates that the cell cycle is disordered and the cells continue to proliferate. p16 alone as the identification standard is required combining morphology and detected simultaneously with Ki67. The results are more reliable.
However, unlike traditional red-brown staining, this reagent employs yellow and cyan blue, making the results easier to interpret. Two positive staining results appear green, making them easier to identify than red or brown stains. This reagent can significantly enhance your diagnostic efficiency and improve diagnostic accuracy.
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Criteria for Kit Identification
Positive refers to observing the same target sample under a microscope, which can be seen single or multiple cells of the cytoplasm stained yellow and the nucleus of the same cell stained cyan blue, that is, the same cell p16 and Ki67 are expressed simultaneously.
Negative refers to observing the same target sample under a microscope, which can not be seen single or multiple cells of the cytoplasm stained yellow and the nucleus of the same cell stained cyan blue, that is, the same cell has only p16 expression or only Ki67 expression, or two indicators.
The fully automated immunocytochemistry staining machine, paired with our proprietary fully automated scanner CNT160 and AI-assisted diagnostic system, forms a comprehensive early cervical cancer screening and diagnostic system.
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