+1-4012094076
info@celnovte.com
En
EN CN
Home News & Event

CELNOVTE IHC Secondary Antibody Help High-level Scientific Research!

2022-08-30

By snt_manager

    Share to:

The discovery and use of antibodies in life science research is critical to many advances in all disease areas. In antibody research, the use of tissue fixation to specifically identify protein expression drives further advances in clinical pathology. Immunohistochemistry (IHC) is a key technique that can be used to differentiate between normal and diseased cells and to help uncover potential approaches for disease diagnosis and treatment.

Xenograft rat/mouse model

The construction of rat/mouse tumor models is the basis for research topics such as oncology, immunology, safety evaluation of drugs and biological products, and efficacy screening.

Immunohistochemical detection of tumor tissue in tumor-bearing animal models can more truly reflect the molecular mechanism of tumor occurrence and development.

However, since the tumor cells in the animal model are of human origin, the surrounding blood vessels, stromal cells, etc. are of mouse origin.

If a certain indicator in human tumor cells is detected, and the commonly used secondary antibody in immunohistochemistry is often goat anti-mouse, it is easy to compare with The murine cells in the tissue produce non-specific staining, which affects the judgment of the experimental results.

“We use high-quality standard development, and produce high-specificity research secondary antibodies through specific serum cross-adsorption, to help researchers to carry out experiments smoothly and obtain ideal results”

What is cross adsorption?

Preadsorption (also known as cross-adsorption) is an additional purification step used to increase the specificity of the antibody.

The solution containing the secondary antibody is passed through a matrix column containing immobilized serum proteins obtained from potentially cross-reactive species. Non-specific secondary antibodies will remain in the column, while highly specific secondary antibodies will pass through.

Pre-adsorbed secondary antibodies can effectively avoid non-specific staining!

Product 1 HRP Secondary Antibody (Goat Anti-Rabbit) Kit

Scenes Scene 1
Species Rat/Mouse tissue
Primary antibody type Rabbit anti-human
Recommend Kit HRP Secondary Antibody (Goat Anti-Rabbit) Kit

Product 2 HRP Secondary Antibody (Goat Anti-Mouse) Kit

Scenes Scene 2
Species Mouse tissue
Primary antibody type Mouse anti-human
Recommend Kit HRP Secondary Antibody (Goat Anti-Mouse) Kit

Product 3 HRP Secondary Antibody (Mouse/Rabbit Universal) Kit

Scenes Scene 3 Scene 4
Species Human tissue Human tissue
Primary antibody type Rabbit anti-human Mouse anti-human
Recommend Kit HRP Secondary Antibody (Mouse/Rabbit Universal) Kit

Summary


Related News

0