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IMMUNOHISTOCHEMISTRY
MICROSTACKER™ DETECTION SYSTEMS
MicroStacker™ Polymer Detection Kit Universal 
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MicroStacker™ Polymer Detection Kit Universal
MicroStacker™ Polymer Detection System Universal is a biotin-free, polymeric horseradish peroxidase (HRP)- secondary antibody conjugate system for the detection of mouse and rabbit primary antibody on formalin-fixed, paraffin-embedded (FFPE) tissues in an immunohistochemistry (IHC) procedure. The innovative MicroStacker™ technology allows well-controlled layered stacking of antibodies and peroxidase enzymes on a micro-polymer scaffold. This technique results in a compact polymeric structure that easily penetrates to all cellular compartments, which provides superior sensitivity compared to other conventional HRP polymers with bulky dextran backbones. Plus, the system utilizes F(ab’) fragments of IgG secondary antibody instead of the whole IgG, which avoids the background caused by non-specific binding of whole IgG to endogenous Fc receptors.
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Product Features
- Known Applications
Immunohistochemistry (formalin-fixed paraffin-embedded tissues)
- Scheme
- Recommended Protocol
Deparaffination: Deparaffination slides in xylene. Hydrate slides in a series of graded alcohols to water.
Peroxidase Block: Block for 5mins with Peroxidase Blocking Reagents. Rinse 2 times with wash buffer for 5mins each.
Antigen Retrieval Protocol: Please refer to the respective primary antibody data sheet for recommended antigen retrieval protocol.
Primary Antibody: Apply 2 drops (100ul) or as much as needed of ready-to-use primary antibody to cover the specimen. Incubate for 30-60mins at RT. Rinse 2 times with wash buffer for 5mins each.
Detection Polymer: Apply 2 drops (100ul) or as much as needed of anti-Mouse/Rabbit HRP-Polymer to cover the specimen. Incubate for 30mins at RT. Rinse 2 times with wash buffer for 5mins each.
DAB Chromogen: Prepare ready-to-use DAB substrate solution. Add DAB Chromogen to DAB Substrate Buffer and mix the two solutions in a 1:20 ratio with the volume determined by the number of slides to stain. In general, 100ul of ready-to-use substrate solution is enough to cover one tissue slide.
Incubate for 5 mins at RT with the ready-to-use substrate solution. Rinse slides with deionized water.
Counterstain: Counterstain with hematoxylin. Rinse slides with deionized water.
Note:
Use TBS-T or PBS-T as wash buffer.
Storage:
Store at 2°C to 8°C, do not freeze.
Data reviews: side-by-side comparison
Manual Staining Procedure：
Primary antibody incubation: RT, 60min
Secondary antibody incubation: RT, 30min
DAB incubation：5min
Detection polymers in comparison:
MicroStackerTM Polymer Detection Kit, Universal
DAKO REAL EnVision Detection System
Experimental Results:
Breast (Her-2 / rabbit monoclonal antibody)
REAL EnVision Detection System
MicroStackerTM Polymer Detection, Universal
Ovary (CK5 / rabbit monoclonal antibody)
REAL EnVision Detection System
MicroStackerTM Polymer Detection, Universal
Specification
|
Code |
Product Name |
Components |
Specification |
|
SD3100 |
MicroStackerTM Polymer Detection Kit, Universal (3 Components) |
MicroStackerTM Anti-Mouse/Rabbit HRP-Polymer |
100ml(1000T) |
|
20*DAB Chromogen |
5ml |
||
|
DAB Substrate Buffer |
100ml |
||
|
MicroStackerTM Anti-Mouse/Rabbit HRP-Polymer |
30ml (300T) |
||
|
20*DAB Chromogen |
1.5ml |
||
|
DAB Substrate Buffer |
30ml |
||
|
MicroStackerTM Anti-Mouse/Rabbit HRP-Polymer |
10ml (100T) |
||
|
20*DAB Chromogen |
1ml |
||
|
DAB Substrate Buffer |
10ml |
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