[RX] MicroStacker™ Mouse-on-Mouse HRP Polymer
The MicroStacker™ technology allows well-controlled layered stacking of F(ab’) fragments of IgG secondary antibody and peroxidase enzymes on a micro-polymer scaffold, which results in a compact polymeric structure with superior sensitivity compared to other conventional HRP polymers with bulky dextran backbones. This system avoids the use of streptavidin and biotin, and therefore eliminates non-specific staining as a result of endogenous biotin. Herein, the Mouse-on-Mouse Polymer comprises of specially engineered anti-Mouse secondary antibody to eliminate the high backgrounds caused by endogenous mouse antibodies in mouse tissues.
Product Feature
Optimized for Mouse-on-Mouse IHC
A biotin-free HRP polymer detection system designed for the reliable detection of mouse primary antibodies on mouse tissue sections.
No Blocking Required
Specially engineered anti-mouse secondary antibodies reduce interference from endogenous mouse IgG, eliminating the need for an additional mouse IgG blocking step and simplifying the staining workflow.
Low Background and High Specificity
The biotin-free design avoids background staining caused by endogenous biotin. Fab’ fragments are used instead of whole IgG secondary antibodies to reduce non-specific binding to endogenous Fc receptors, delivering cleaner and more specific staining.
Enhanced Sensitivity
MicroStacker™ technology enables orientation-controlled attachment of Fab’ fragments to a compact poly-HRP core. This helps preserve antibody binding activity during conjugation and enhances signal intensity.
Consistent Staining Performance
The compact micro-polymer structure supports effective penetration into tissue sections and reliable detection of nuclear, cytoplasmic, and membranous antigens.
Superior Polymer Design
Compared with conventional Mouse-on-Mouse methods and bulky dextran-based HRP polymers, MicroStacker™ provides a simpler workflow, lower background, and sensitive, reproducible staining results.

Experimental Group
MicroStackerTM Mouse-on-Mouse Polymer Detection Kit
Comparison with Detection Polymers
Compared with international leading brand detection systems.
Staining Results
Mouse Spleen – SMA, 1% PEG

Mouse Kidney – SMA, 1% PEG

Specification
| Cat# | Product Name | Size | Components |
| CSM1004 | MicroStacker™ Mouse-on-Mouse HRP Polymer | 20mL, 100mL, 1000mL | Anti-mouse HRP Polymer |
| CSK2001 | MicroStacker™ Mouse-on-Mouse HRP Polymer Detection Kit | 200T, 1000T | 1. Anti-mouse HRP Polymer 2. DAB Substrate 3. DAB Buffer |
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Specification
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