Home Product IMMUNOHISTOCHEMISTRY Multiplex immunohistochemical, mIHC kit

Multiplex immunohistochemical, mIHC kit

mIHC kits

Multiplex immunohistochemistry (IHC) is a technique used in pathology and research to simultaneously detect and visualize multiple different biomarkers within a single tissue section.This technique is often used to investigate the expression of different proteins, their interactions, and their spatial distribution within a tissue sample.

Chromogenic mIHC is one of the alternatives, and even if this technique is much easier to be used routinely, it is limited by the use of 4 antibodies on the same slide. In addition, fluorescence reveals membrane co-localizations (in the membrane or the nucleus), which is more difficult to obtain with the latter technique. Nevertheless, the use of multiple antibodies (mixed or used step by step) was restricted to the specificity of the primary and the risk of false positivity due to cross reactivity between them. Until recently, the single-parametric or even multiparametric (double or triple) staining, revealed by chromomeric or fluorescent staining, were most often read and interpreted directly by the researchers。

It’s taken as the mandatory tool for tumor microenvironment research.

 

Specification

4-color mIHC FM2004 20T,50T,100T Universal detection system;
FT1520, FT1570,FT1690,FTDAPI
5-color mIHC FM2005 20T,50T,100T Universal detection system;
FT1520, FT1570,FT1620, FT1690,FTDAPI
6-color mIHC FM2006 20T,50T,100T Universal detection system;
FT1480, FT1520, FT1570,FT1620, FT1690,FTDAPI
7-color mIHC FM2007 20T,50T,100T Universal detection system;
FT1480, FT1520, FT1570,FT1620, FT1690,FT1780, FTDAPI
Single color TSA stain-480 FT1480 20T,50T,100T 480 stains; Amplification solution
Single color TSA stain-520 FT1520 20T,50T,100T 520 stains; Amplification solution
Single color TSA stain-540 FT1540 20T,50T,100T 540 stains; Amplification solution
Single color TSA stain-570 FT1570 20T,50T,100T 570 stains; Amplification solution
Single color TSA stain-620 FT1620 20T,50T,100T 620 stains; Amplification solution
Single color TSA stain-650 FT1650 20T,50T,100T 650 stains; Amplification solution
Single color TSA stain-690 FT1690 20T,50T,100T 690 stains; Amplification solution
Single color TSA stain-780 FT1780 20T,50T,100T 780 stains; Amplification solution A&B
Single color stain-DAPI FTDAPI 20T,50T,100T DAPI Stain Ready-to-Use
AntigenRetrieval Buffer(CBS) SD2001 2.0L Acid Antigen Repair
AntigenRetrieval Buffer(EDTA 50X) SD2003 50mL Alkaline antigen repair
TBS concentrated washing buffer (10X) SD2004 50mL,2.5L  
Tissue autofluorescence quencher FM1101 2mL,5mL,10mL  
Anti-fluorescence quencher sealer FM1102 2mL,5mL,10mL  

More Info

Allows protocol runs on in-house automation-CNT330

6-8 markers detection simultaneously 

No species restrictions

High Sensitivity and Specificity

 

 

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