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![[Manual] MicroStacker™ Polymer Detection Kit, Enhanced, 2-Step](https://www.celnovte.com/wp-content/uploads/2020/08/1-1.gif "[Manual] MicroStacker™ Polymer Detection Kit, Enhanced, 2-Step")
![[Manual] MicroStacker™ Polymer Detection Kit, Enhanced, 2-Step](https://www.celnovte.com/wp-content/uploads/2020/08/图片10.png "[Manual] MicroStacker™ Polymer Detection Kit, Enhanced, 2-Step")
[Manual] MicroStacker™ Polymer Detection Kit, Enhanced, 2-Step
MicroStacker™ Polymer Detection System is a biotin-free, polymeric horseradish peroxidase (HRP)- secondary antibody conjugate system for the detection of mouse and rabbit primary antibody on formalin-fixed, paraffin-embedded (FFPE) tissues in an immunohistochemistry (IHC) procedure. The innovative MicroStacker™ technology allows well-controlled layered stacking of antibodies and peroxidase enzymes on a micro-polymer scaffold. This technique results in a compact polymeric structure that easily penetrates to all cellular compartments. Plus, the system utilizes F(ab’) fragments of IgG secondary antibody instead of the whole IgG, which avoids the background caused by non-specific binding of whole IgG to endogenous Fc receptors. Compared to the MicroStacker™ Polymer Detection System, Universal, the Enhanced kit comes with the HRP polymer at a higher degree of polymerization, thus higher sensitivity.
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MicroStacker™ Polymer Detection Kit, Enhanced – 2-Step IHC Detection System
MicroStacker™ Enhanced is a biotin-free polymer-based HRP secondary antibody detection system optimized for formalin-fixed paraffin-embedded (FFPE) tissue sections. It’s designed to detect mouse and rabbit primary antibodies in IHC workflows.
Compared to the standard MicroStacker™ Universal kit, the Enhanced version features a higher degree of polymerization, offering greater sensitivity and improved signal intensity.
Product Highlights
- Biotin-free, polymeric HRP-conjugated secondary antibody
- Uses F(ab’) fragment to reduce background staining
- Compact polymer structure allows deep tissue penetration
- Ideal for mouse-on-mouse detection
- Enhanced signal intensity for low-expression targets
Recommended Manual IHC Protocol
- Deparaffinization: Xylene and graded alcohols to water
- ペルオキシダーゼ・ブロック 5 min block, rinse with wash buffer
- Antigen Retrieval: Follow primary antibody datasheet
- Primary Antibody Incubation: 30–60 min at RT
- Detection Polymer Incubation: 30 min at RT
- DAB Development: 5 min using ready-to-use solution
- 反対票： ヘマトキシリン
Use TBS-T or PBS-T as wash buffer. Store reagents at 2–8°C. Do not freeze.
Side-by-Side Comparison: Experimental Results
Part 1: HER2 (Rabbit mAb) on Breast Tissue
- REAL EnVision™ Detection System
- MicroStacker™ Universal
- MicroStacker™ Enhanced
REAL EnVision™
MicroStacker™ Universal
MicroStacker™ Enhanced
Part 2: D2-40 (Mouse mAb) on Esophagus Tissue
- REAL EnVision™
- EnVision™ Flex + Mouse Linker
- MicroStacker™ Enhanced
REAL EnVision™
EnVision™ Flex + Mouse Linker
MicroStacker™ Enhanced
仕様
| Cat # | 製品名 | Size | コンポーネント |
|---|---|---|---|
| SD3206 | MicroStacker™ Polymer Detection Kit, Enhanced, 2-Step (4 components) | 200T, 1000T |
1 Linker,Rabbit-anti-Mouse 2 Anti-Rabbit HRP-Polymer 3 DAB Chromogen (20x) 4 DAB Substrate Buffer |
| SD3207 | MicroStacker™ Polymer Detection Kit, Enhanced, 2-Step (5 components) | 200T, 1000T |
1 Linker,Rabbit-anti-Mouse 2 Anti-Rabbit HRP-Polymer 3 DAB Chromogen (20x) 4 DAB Substrate Buffer 5 Peroxidase Block |
| SD3208 | MicroStacker™ Polymer Detection Kit, Enhanced, 2-Step (6 components) | 200T, 1000T |
1 Linker,Rabbit-anti-Mouse 2 Anti-Rabbit HRP-Polymer 3 DAB Chromogen (20x) 4 DAB Substrate Buffer 5 Peroxidase Block 6 Hematoxylin |
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