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Microstacker™マウスオンマウスポリマー検出キット
The MicroStacker™ technology allows well-controlled layered stacking of F(ab’) fragments of IgG secondary antibody and peroxidase enzymes on a micro-polymer scaffold, which results in a compact polymeric structure with superior sensitivity compared to other conventional HRP polymers with bulky dextran backbones. This system avoids the use of streptavidin and biotin, and therefore eliminates non-specific staining as a result of endogenous biotin. Herein, the Mouse-on-Mouse Polymer comprises of specially engineered anti-Mouse secondary antibody to eliminate the high backgrounds caused by endogenous mouse antibodies in mouse tissues.
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- Scheme
Staining Procedure：
Primary antibody incubation: RT, 30-60min
Secondary antibody incubation: RT, 15min
DAB incubation：5min
Experimental group:
MicroStackerTM Mouse-on-Mouse
Detection polymers in comparison:
International Famous Brand
Staining Photos:
Mouse spleen – SMA-1%-PEG:
Mouse kidney – SMA-1%-PEG:
仕様
|
Code |
Product Name |
Components |
仕様 |
|
RS4000 |
MicroStackerTM Mouse-on-Mouse Polymer Detection Kit (3 Components) |
MicroStackerTM Anti-Rabbit HRP-Polymer |
100ml (1000T) |
|
20*DAB Chromogen |
5ml |
||
|
DAB Substrate Buffer |
100ml |
||
|
MicroStackerTM Anti-Rabbit HRP-Polymer |
30ml (300T) |
||
|
20*DAB Chromogen |
1.5ml |
||
|
DAB Substrate Buffer |
30ml |
||
|
MicroStackerTM Anti-Rabbit HRP-Polymer |
20ml (200T) |
||
|
20*DAB Chromogen |
1ml |
||
|
DAB Substrate Buffer |
20ml |
||
|
RS4001 |
MicroStackerTM Mouse-on-Mouse Polymer Detection Kit (4 Components) |
Peroxidase Blocking Reagents |
100ml |
|
MicroStackerTM Anti-Rabbit HRP-Polymer |
100ml (1000T) |
||
|
20*DAB Chromogen |
5ml |
||
|
DAB Substrate Buffer |
100ml |
||
|
Peroxidase Blocking Reagents |
20ml |
||
|
MicroStackerTM Anti-Rabbit HRP-Polymer |
20ml (200T) |
||
|
20*DAB Chromogen |
1ml |
||
|
DAB Substrate Buffer |
20ml |
||
|
RS4002 |
MicroStackerTM Mouse-on-Mouse Polymer Detection Kit (5 Components) |
Peroxidase Blocking Reagents |
100ml |
|
MicroStackerTM Anti-Mouse/Rabbit HRP-Polymer |
100ml (1000T) |
||
|
20*DAB Chromogen |
5ml |
||
|
DAB Substrate Buffer |
100ml |
||
|
Hematoxylin |
100ml |
||
|
Peroxidase Blocking Reagents |
20ml |
||
|
MicroStackerTM Anti-Mouse/Rabbit HRP-Polymer |
20ml (200T) |
||
|
20*DAB Chromogen |
1ml |
||
|
DAB Substrate Buffer |
20ml |
||
|
Hematoxylin |
20ml |
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