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Elastic Fiber Staining Kit

Elastic Fibers are widely distributed in fibrous connective tissue, especially concentrated in the ligament of arcus vertebrae, vocal cords, alveolar wall, arterial wall and elastic cartilage. Therefore, elastic fibers staining is widely used in pathological diagnosis, such as tumor vein infiltration, serous membrane infiltration to diagnose early tumor infiltration. The hyperplasia disease of skin elastic fiber is often used in elastic fiber. In HE staining of the skin, elastic fibers are similar to collagen fiber and they are stained red in different shades and more difficult to distinguish them. Only by special staining of elastic fiber can support this distinguish.

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製品機能

Staining Principle

The staining principle of elastic fiber stain (modified Gomori aldehyde fuchsin method) is that the mature aldehyde fuchsin has a strong affinity for special proteins and mucopolysaccharides containing sulfate groups, which combines well with elastic fiber. The staining solution also showed mast cell particles, lipofuscin, eosinophils, as so on.

製品機能

a)Bright staining; The elastic fibers are clearly separated from the surrounding background

b)No precipitates and insoluble substances in each component reagent; stable

仕様

Components

Name		Main Component
Solution A: potassium permanganate solution		potassium permanganated
Solution B: oxalic acid solution		Oxalic acid
Solution C: victoria blue solution		victoria blue, resorcinol
Solution D: Van Gieson/nuclear fast red solution		acid fuchsin/nuclear fast red

詳細

Staining Results : acidic mucus material is blue, neutral mucus material is red, mixed mucus material is blue-violet, and cell nuclei are light blue